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Biophysics and the Challenges of Emerging Threats (NATO Science for Peace and Security Series B: Physics and Biophysics)

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 |  Springer |  2009-06-04 |  2009-06-05 | Original language:English | PDF # 1 |  9.25 x.43 x6.10l,.60 | File type: PDF | 179 pages

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Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for...

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