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Method development and validation of anti-histaminic drug in plasma: Determination of fexofenadine in human plasma using Ultra Performance Liquid Chromatography

Mr.Maulikkumar Amin, Dr.Deepti Jain, Dr.M.M.Patel




[PDF.us94] Method development and validation of anti-histaminic drug in plasma: Determination of fexofenadine in human plasma using Ultra Performance Liquid Chromatography

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 |  2011-08-18 | Original language:English | PDF # 1 |  8.66 x.18 x5.91l,.27 | File type: PDF | 76 pages

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A selective, rapid and sensitive reverse phase ultra- performance liquid chromatography method was developed for the quantitative determination of fexofenadine in human plasma. With carbamazepine as internal standard, sample pretreatment involved a one-step extraction with ethyl acetate from 980µl plasma. The sample was analyzed using 10mM KH2PO4 buffer pH 2.5 and acetonitrile (70:30 v/v) as mobile phase. Chromatographic separation was achieved on an ACQUI...

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